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Image Search Results
Journal:
Article Title: Development of a novel self micro-emulsifying drug delivery system (SMEDDS) for reducing HIV protease inhibitor-induced intestinal epithelial barrier dysfunction
doi: 10.1021/mp100003r
Figure Lengend Snippet: A. IEC-6 cells stably transfected with pSEAP plasmid were treated with vehicle control (DMSO), ritonavir (RTV, 15 µM) in the absence or presence of individual antioxidant, vitamin C (VC 15 µg/ml) or, vitamin E (VE 20 µg/ml), or ethyl linoleate (EL, 20 µg/ml) or oleic acid (OA, 10 µg/ml) or TG (100 nM) for 24 h. Activity of SEAP was measured as described under “Methods” and expressed as percent of control. Values are mean ± SD of 3 independent experiments. Statistical significance relative to vehicle control, * p< 0.05 or relative to RTV, #p<0.05. B. Representative immunoblots against CHOP and lamin B from the nuclear extracts of IEC-6 cells treated with ritonavir (RTV, 15 µM) in the absence or presence of individual antioxidant, vitamin C (VC 15 µg/ml) or, vitamin E (VE 20 µg/ml), or ethyl linoleate (EL, 20 µg/ml or 40 µg/ml) or oleic acid (OA, 10 µg/ml) for 4 h. Lamin B was used as a loading control of nuclear protein. Relative protein levels of CHOP from three independent experiments. Statistical significance relative to control, *p < 0.05 or relative to RTV, #p<0.05.
Article Snippet: The SEAP enzyme activity was measured by chemiluminescent assays using
Techniques: Stable Transfection, Transfection, Plasmid Preparation, Control, Activity Assay, Western Blot
Journal:
Article Title: Development of a novel self micro-emulsifying drug delivery system (SMEDDS) for reducing HIV protease inhibitor-induced intestinal epithelial barrier dysfunction
doi: 10.1021/mp100003r
Figure Lengend Snippet: A. IEC-6 cells stably transfected with pSEAP plasmid were treated with vehicle control (DMSO), ritonavir in DMSO (RTV DMSO, 15 µM), ritonavir SMEDDS (RTV ME, 15 µM) or Norvir® (15 µM), or TG (25 nM) for 24 h. Activity of SEAP was measured as described under “Methods” and expressed as percent of control. Values are mean ± SD of 3 independent experiments. Statistical significance relative to vehicle control, * p< 0.05, or relative to RTV DMSO, #p<0.05, B. Representative immunoblots against CHOP and lamin B from the nuclear extracts of IEC-6 cells treated with vehicle control (DMSO), ritonavir in DMSO (RTV DMSO, 15 µM), ritonavir SMEDDS (RTV ME, 15 µM) or Norvir® (15 µM), or TG (25 nM) for 4 h. Lamin B was used as a loading control of nuclear protein. Relative protein levels of CHOP from three independent experiments were analyzed. Statistical significance relative to control, *p < 0.05 or relative to RTV DMSO, #p<0.05.
Article Snippet: The SEAP enzyme activity was measured by chemiluminescent assays using
Techniques: Stable Transfection, Transfection, Plasmid Preparation, Control, Activity Assay, Western Blot